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¹ Batı Akdeniz Agricultural Research Institute, Antalya
² Akdeniz University Agricultural Faculty Agricultural Biotechnology Department, Antalya
³ Antalya Tarim, Antalya
⁴ Ege University Agricultural Faculty Horticulture Department, İzmir
⁵ Cukurova University Agricultural Faculty Plant Protection Department, Adana
⁶ Antalya Agricultural Quarantine Directorate, Antalya DOI : 10.16882/derim.2016.07605 Viewed : 1738 - Downloaded : 862 Bacterial canker is one of the most important diseases causing economic yield loss in tomato production areas in the world. The best way to control for this disease is to use resistant varieties. However, there are few studies on variety breeding studies of this disease compared with other disease resistant breeding studies. In this study we aimed to improve inbred lines carrying bacterial canker resistance genes to use in the breeding of resistant varieties. Susceptible inbred line AK1 (S. esculentum) and resistant LA2157 (S. peruvianum) were crossed. Embryo rescue and ovule culture techniques were applied in 30 fruits to get F1 hybrids. Rescued embryos and immature ovules were cultured in petri dishes containing solidified MS medium without hormone. 30 healty embryos were excised and cultured from 30 fruits 27-61 day old (1 embryo fruit^-1) in embryo rescue method. The two surviving plants from acclimatization were transferred to the greenhouse to get their BC1 progenies. Resistance tests were performed according to the stem inoculation method in the BC1 and BC2 progenies. The mixture of 14 aggressive Turkish Cmm strains were used to confirm the resistance. The plants were valued by 0-4 scale. Plants with 0 and 1 scale values were used to obtain next progenies. A total of 80 BC3 resistant progenies were transferred to our variety breeding programme. Keywords : Tomato, Clavibacter, Embryo rescue, Breeding