Horticultural Studies (HortiS) 2003, Vol 20, Num, 1     (Pages: 43-47)

DETERMINATION OF RAPDS MOLECULAR MARKERS FOR CITRUS SPECIES

MÜNEVVER GÖCMEN 1 ,CAHİT ÇAKIR 2 ,İLKNUR POLAT 1

1 Narenciye ve Seracılık Araştırma Enstitüsü, 07100, Antalya/TÜRKİYE
2 Akdeniz Üniversitesi, Ziraat Fakültesi, Antalya/TÜRKİYE
Viewed : 1453 - Downloaded : 781 In this study, RAPD molecular technique was used in order to determine genetic similarity and obtain molecular markers for some citrus species (mandarin, orange, grapefruit and Flying Dragon (a kind of Poncirus spp.). For this aim, 20 RAPD primers (OP-F), each of which consisted of 10 nucleotid bases, were used. While a total of 125 polymorphic DNA bands were obtained by 16 primers, 40 monomorphic bands were found by 16 primers and no DNA synthesis was materialized by 4 of that. Each of four species was established as different at the DNA level. The rate of the less genetic similarity was found as 21% between Flying Dragon and `Pixie` species while the rate of the most genetic similarity was determined as 56% between `Pixie` (mandarin) and `Navelina` (orange). Keywords : Citrus, RAPD, Molecular Markers